在GridView添加一个模板列：
<asp:TemplateField>
                <HeaderTemplate>
                    <asp:CheckBox ID="cbSelAll" runat="server" Text="全选" AutoPostBack="True" OnCheckedChanged="cbSelAll_CheckedChanged" />
                </HeaderTemplate>
                <ItemTemplate>
                    <asp:CheckBox ID="cbSel" runat="server" />
                </ItemTemplate>
            </asp:TemplateField> 　　用法： protected void cbSelAll_CheckedChanged(object sender, EventArgs e)
    {
        CheckBox cbAll = (CheckBox)sender;
        if (cbAll.Text == "全选")
        {
            foreach (GridViewRow gvr in GridView1.Rows)
            {
                CheckBox cbSel = (CheckBox)gvr.Cells[0].FindControl("cbSel");
                cbSel.Checked = cbAll.Checked;
            }
        }
    }
foreach (GridViewRow gvr in GridView1.Rows)
        {
            CheckBox cbSel = (CheckBox)gvr.Cells[0].FindControl("cbSel");
            if (cbSel.Checked == false)
            {  

                //...
            }
        } 

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